, var_names='velocity_genes', n_top_genes=None, max_iter=10, assignment_mode='projection', t_max=None, fit_time=True, fit_scaling=True, fit_steady_states=True, fit_connected_states=None, fit_basal_transcription=None, use_raw=False, load_pars=None, return_model=None, plot_results=False, steady_state_prior=None, add_key='fit', copy=False, n_jobs=None, backend='loky', **kwargs)

Recovers the full splicing kinetics of specified genes.

The model infers transcription rates, splicing rates, degradation rates, as well as cell-specific latent time and transcriptional states, estimated iteratively by expectation-maximization.
data: AnnData

Annotated data matrix.

var_names: str, list of str (default: ‘velocity_genes’)

Names of variables/genes to use for the fitting. If var_names=’velocity_genes’ but there is no column ‘velocity_genes’ in adata.var, velocity genes are estimated using the steady state model.

n_top_genes: int or None (default: None)

Number of top velocity genes to use for the dynamical model.

max_iter:int (default: 10)

Maximal iterations in the EM-Algorithm.

assignment_mode: str (default: projection)

Determined how times are assigned to observations. If projection, observations are projected onto the model trajectory. Else uses an inverse approximating formula.

t_max: float, False or None (default: None)

Total range for time assignments.

fit_scaling: bool or float or None (default: True)

Whether to fit scaling between unspliced and spliced.

fit_time: bool or float or None (default: True)

Whether to fit time or keep initially given time fixed.

fit_steady_states: bool or None (default: True)

Whether to explicitly model and fit steady states (next to induction/repression)

fit_connected_states: bool or None (default: None)

Restricts fitting to neighbors given by connectivities.

fit_basal_transcription: bool or None (default: None)

Enables model to incorporate basal transcriptions.

use_raw: bool or None (default: None)

if True, use .layers[‘sliced’], else use moments from .layers[‘Ms’]

load_pars: bool or None (default: None)

Load parameters from past fits.

return_model: bool or None (default: True)

Whether to return the model as :DynamicsRecovery: object.

plot_results: bool or None (default: False)

Plot results after parameter inference.

steady_state_prior: list of bool or None (default: None)

Mask for indices used for steady state regression.

add_key: str (default: ‘fit’)

Key to add to parameter names, e.g. ‘fit_t’ for fitted time.

copy: bool (default: False)

Return a copy instead of writing to adata.

n_jobs: int or None (default: None)

Number of parallel jobs.

backend: str (default: “loky”)

Backend used for multiprocessing. See joblib.Parallel for valid options.


adata (AnnData) – Updated AnnData with inferred parameters added to .var if copy=True. The inferred parameters are the transcription rates fit_alpha, splicing rates fit_beta, degradation rates fit_gamma, switching times fit_t_, variance scaling factor for unspliced and spliced counts, model likelihoods fit_likelihood, and the scaling factor to align gene-wise latent times to a universal latent time fit_alignment_scaling.